Histopathological evaluation of ocular microsporidiosis by different stains

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Histopathological evaluation of ocular microsporidiosis by different stains

BACKGROUND There is limited data on comparing stains in the detection of microsporidia in corneal biopsies. Hence we wanted to evaluate various stains for their ability to detect microsporidia in corneal tissue sections. METHODS Four cases diagnosed with microsporidiosis on Hematoxylin and Eosin and Periodic Acid Schiff's stained sections of the corneal button between January 2002 and Decembe...

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Use of different stains for microscopic evaluation of corneal scrapings for diagnosis of microsporidial keratitis.

Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H(2)SO(4), cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.

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ژورنال

عنوان ژورنال: BMC Clinical Pathology

سال: 2006

ISSN: 1472-6890

DOI: 10.1186/1472-6890-6-6